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目的 :评价中药补肾益精方水提物和醇提物对化学成分指标含量以及对绝经后骨质疏松症大鼠模型骨矿密度的影响 ,考察化学成分指标与药效的相关性 ,探讨复方有效组分。方法 :分别采用水提和醇提工艺制备补肾益精方的提取物 ,用HPLC法测定淫羊藿苷和柚皮苷含量 ,并观察两种提取物预防性给药对切除卵巢诱导的绝经后骨质疏松症大鼠模型的作用。结果 :水提物和醇提物均具有抗骨质疏松作用 ,且水提物的作用略强于醇提物 ,而水提物中的淫羊藿苷和柚皮苷的含量低于醇提物 ,淫羊藿苷和柚皮苷的含量与复方抗骨质疏松作用非正性相关。结论 :补肾益精方以水提为佳 ,复方中水溶性成分和醇溶性成分都具有抗骨质疏松的作用 ,复方的提取工艺筛选以药效为指标较妥。
OBJECTIVE: To evaluate the effect of Bushen Yijing Recipe aqueous extract and alcohol extract on the content of chemical constituents and bone mineral density in postmenopausal osteoporosis rat models, and to examine the correlation between chemical composition indexes and efficacy, and to explore the Effective component. METHODS: Extracts of Bushen Yijing Recipe were prepared by water extraction and ethanol extraction respectively. The contents of icariin and naringin were determined by HPLC, and the preventive administration of the two extracts was observed after ovariectomy-induced menopause. The role of a rat model of osteoporosis. RESULTS: Both aqueous extracts and alcohol extracts had anti-osteoporosis effects, and the effect of aqueous extract was slightly stronger than that of alcohol extracts, while the content of icariin and naringin in the aqueous extract was lower than that of alcohol extracts. The content of icariin and naringin was not positively related to the anti-osteoporosis effect of the compound. Conclusion : Bushenyijing prescription is better by water extraction. Both water-soluble and alcohol-soluble components of the compound have anti-osteoporosis effect. The selection of compound extraction process is better based on drug efficacy.