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[目的]研究抗松针褐班病湿地松子代组培苗瓶内菌根化。[方法]对湿松地再生植株离体条件下菌根的形成进行了研究,并对再生植株的生长状况进行了观察。[结果]菌根真菌培养基质和接种量对离体条件下菌根的形成有较大影响。湿地松丛生芽诱导出根原基后,以转接到珍珠岩为培养基质接种彩色豆马勃为最佳,有利于菌根的形成;在珍珠岩接种彩色豆马勃为2个菌块时,菌根化率高达84.4%,形成的二叉分枝状短根最多,平均每条主根上形成12.49条;菌根的形成提高了再生植株的驯化移栽成活率,菌根化再生植株在植物生长室长势良好,根系发达。[结论]为提高抗松针褐班病湿地松组培再生植株的成活率提供了依据。
[Objective] The research aimed to study the mycorrhizal effect of Pinus tabulaeformis tissue culture seedling on pine needles. [Method] The formation of mycorrhizae in vitro under the condition of regenerated plants was studied, and the growth of regenerated plants was observed. [Result] Mycorrhizal fungi culture medium and inoculation amount had a great influence on mycorrhiza formation in vitro. After the roots of Pinus elliottii were induced to root, the seedlings were inoculated with perlite as the culture medium, which was the best for the formation of mycorrhizal fungi. When perlite was inoculated with 2 fungi, The rate of mycorrhiza up to 84.4%, the formation of the largest bifurcated branched short stalk, an average of 12.49 per main root formation; mycorrhizal formation increased regeneration of domesticated transplanting survival rate of mycorrhized regeneration plants in plants Growth chamber grows well, roots developed. [Conclusion] This study provided the basis for improving the survival rate of regenerated tissue culture of Pinus elliottii from pine needles.