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叶绿体是绿色植物把光能转化为化学能的重要细胞器.目前,多种植物的叶绿体基因组序列已经获得,对叶绿体内发生的各种生物学过程人们也已经有相当深入的了解,但对叶绿体基因编码蛋白质的表达还所知甚少.用蛋白质印迹实验系统地检测了15个叶绿体基因编码蛋白质在水稻叶片不同生长时期的表达.其中7个与光合作用相关蛋白质的表达具有相似的模式,其表达量随叶片生长而增加,一般在孕穗期、开花期达到最高峰,在成熟期叶片下降,这种模式与水稻生长对光合作用的需求有明显相关性.4个与DNA复制相关的RNA聚合酶在苗期叶片中表达量达到最高,说明这些聚合酶在较早时期发挥作用.4个NADH脱氢酶蛋白质的表达呈2种不同的模式,其中亚基2和4在种子萌发后的早期叶片中就达到最高峰,亚基5和7的最高峰出现在中后期,反映了它们之间功能上的不同.实验结果直观且相对定量地揭示了叶绿体编码蛋白质的表达与叶片生长之间的关联关系,为深入了解其功能提供了重要的线索.
Chloroplasts are important organelles of green plants that convert light energy into chemical energy.Currently, the chloroplast genome sequences of many plants have been obtained, and people have a good understanding of various biological processes occurring in chloroplasts. However, The expression of the encoded proteins was also poorly understood.The expression of 15 chloroplast genes encoding proteins at different growth stages of rice leaves was systematically detected by Western blotting.Among these 7 proteins, The amount increased with leaf growth, and reached its peak at booting stage and flowering stage, and decreased at mature stage. There was a significant correlation between this mode and the demand of photosynthesis for rice growth. Four RNA polymerase related to DNA replication The expression of these NADH dehydrogenase proteins showed two different patterns, among which the subunits 2 and 4 showed the highest expression level in early leaves after seed germination In the peak reached the highest peak of subunits 5 and 7 appear in the late, reflecting the functional differences between them.The experimental results are intuitive The correlation between chloroplast-encoded protein expression and leaf growth was relatively quantitatively revealed, providing important clues for understanding its function.