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目的:观察苏子油和非诺贝特对高脂血症大鼠血脂及阻力血管结构与功能的影响。方法:40只8周龄雄性SD大鼠随机分成5组:正常组(普通饲料+生理盐水)、高脂组(高脂饲料+生理盐水)、非诺贝特组(高脂饲料+非诺贝特100mg.kg-1.d-1)、苏子油小剂量组(高脂饲料+苏子油0.34g.kg-1.d-1)、苏子油大剂量组(高脂饲料+苏子油2.00g.kg-1.d-1),喂养12周后称体重;采血检测各组大鼠血脂、白介素-6(IL-6)水平;HE染色测定肠系膜动脉分支第三级血管的血管壁面积/血管腔面积比(W/L);平衡记录仪记录离体肠系膜第三级动脉血管环对硝普钠反应的敏感性。结果 (:1)饲养12周后,与正常组相比,高脂组和苏子油小剂量组大鼠体重明显增加(P<0.05),而苏子油大剂量组和非诺贝特组无明显变化,且显著低于高脂组(P<0.05);(2)干预12周后,高脂组大鼠的总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、极低密度脂蛋白胆固醇(VLDL-C)及白介素-6(IL-6)均显著高于正常组(P<0.05);苏子油小剂量组和大剂量组均能显著性降低血脂与IL-6(P<0.05),且对于LDL-C、TG,VLDL-C表现出剂量依赖性(P<0.05);苏子油大剂量组血脂及IL-6的水平与非诺贝特组无显著性差异(P>0.05);(3)高脂组大鼠肠系膜三级动脉W/L显著增加(P<0.01),其对硝普钠的敏感性也显著下降(P<0.01),而苏子油呈剂量依赖性的抑制血管壁的增厚(P<0.05),增加肠系膜三级动脉对硝普钠的敏感性(P<0.01),且与非诺贝特组无显著性差异(P>0.05)。结论:苏子油可以改善高脂饲料饲养大鼠的血脂异常,减少炎症因子的产生,抑制阻力血管的管壁增厚,改善血管的舒张功能,且疗效与非诺贝特相当。
Objective: To observe the effects of perilla oil and fenofibrate on the structure and function of blood lipid and resistance blood vessels in hyperlipidemic rats. Methods: Forty eight-week-old male Sprague-Dawley rats were randomly divided into five groups: normal group (normal feed + saline), high fat group (high fat diet + saline) and fenofibrate group (100mg.kg-1.d-1), low-dose group of perilla oil (0.34g.kg-1.d-1 for high fat diet + Perilla seed oil 2.00g.kg-1.d-1) were weighed and fed for 12 weeks. Blood samples were collected for blood lipids and interleukin-6 (IL-6) Of the vessel wall area / vascular lumen area ratio (W / L); balance recorder recorded in vitro mesenteric third-level arterial ring ring sensitivity to sodium nitroprusside reaction. Results: (1) After 12 weeks of feeding, compared with the normal group, the body weight of rats in the high-fat group and the low-dose group of suet oil increased significantly (P <0.05), while the high-fat group and fenofibrate group (P <0.05). (2) After 12 weeks of intervention, total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), triglyceride TG, VLDL-C and IL-6 were significantly higher than those in the normal control group (P <0.05). Both the low-dose group and the high-dose group (P <0.05), and LDL-C, TG, VLDL-C showed a dose-dependent manner (P <0.05). The levels of serum lipids and IL-6 in high-dose group (P> 0.05). (3) The W / L of mesenteric third artery increased significantly in hyperlipidemic rats (P <0.01), and the sensitivity to sodium nitroprusside also decreased significantly (P (P <0.01). However, SUI inhibited the thickening of the vessel wall in a dose-dependent manner (P <0.05) and increased the sensitivity of the mesenteric artery to sodium nitroprusside (P <0.01) No significant difference (P> 0.05). Conclusion: SUI can improve lipid metabolism in rats fed with high-fat diet, reduce the production of inflammatory cytokines, inhibit the wall thickening of resistance vessels and improve the diastolic function of blood vessels, and the curative effect is comparable to fenofibrate.