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为进一步了解和认识CuZnSOD基因的结构和功能,从13个烟草品种及林烟草种的幼叶cDNA中成功扩增出CuZnSOD基因编码区全长序列,并对其进行序列分析。烟草CuZnSOD基因编码序列全长291bp,编码1个96个氨基酸长度的蛋白质,其核苷酸序列关键区域与Genbank中其他植物的CuZnSOD相似性高至94%。遗传进化分析结果表明,烟草CuZnSOD基因与西红柿和马铃薯CuZnSOD基因亲缘关系最近。通过荧光定量PCR对低温弱光胁迫条件下13个烟草品种及林烟草种的CuZnSOD基因表达量进行分析,结果显示在低温弱光胁迫条件下,CuZnSOD基因在13个烟草品种及林烟草种间表达模式存在显著差异。对烟草CuZnSOD基因表达模式的分析,有助于揭示烟草对环境的适应机制及抗氧化的生理机制。
In order to further understand and understand the structure and function of CuZnSOD gene, the full-length coding sequence of CuZnSOD gene was successfully amplified from cDNA of young leaves of 13 tobacco varieties and Nicotiana tobacum L. and sequenced. The coding sequence of tobacco CuZnSOD gene was 291 bp in length, encoding a protein of 96 amino acids in length. The similarity of CuZnSOD with other plants in Genbank was as high as 94%. The results of genetic evolution analysis showed that the tobacco CuZnSOD gene was the most closely related to the CuZnSOD gene of tomato and potato. The quantitative analysis of CuZnSOD gene expression in 13 tobacco cultivars and tobacco varieties under low temperature and low light stress showed that CuZnSOD gene was expressed in 13 tobacco cultivars and forest tobacco cultivars at low temperature and weak light stress There are significant differences in the patterns. The analysis of CuZnSOD gene expression patterns in tobacco helps to reveal the mechanism of adaptation of tobacco to the environment and the physiological mechanism of antioxidation.