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The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H_(11) against gastric cancer cell line. Antigen purified by affinity chromatography and further charac- terized by biochemical and immunological techiques. Results showed that assoiciated antigen of McAb 3H_(11) was mainly located on the membrane of target cells. It was sensitive to heat and digestion of proteases,but resistant to treatment with sodium periodate. Schiff’s reagent staining was negative. SDS-PAGE and IEF- analysis showed that the antigen was a 210 kD molecular weight with pl of 8. 5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the cor- responding antigen of McAb 3H_(11) might be an alkaline protein.
The object of this study is to investigate the properties of gastric cancer associated antigen by McAb3H_11 against gastric cancer cell line. Antigen purified by affinity chromatography and further charac- terized by biochemical and immunological techiques. Results showed that associated antigen of McAb 3H_ (11) was essentially located on the membrane of target cells. It was sensitive to heat and digestion of proteases,but resistant to treatment with sodium periodate. Schiff’s reagent staining was negative. SDS-PAGE and IEF- analysis showed that the antigen was a We concluded that the molecular weight of pl of 8. 5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the cor-requiring antigen of McAb 3H_(11) might be an alkaline protein.