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研究鲜马奶粉对D-半乳糖诱导的氧化损伤小鼠体内抗氧化作用。小鼠随机分为6组—空白对照组(灌胃生理盐水),D-半乳糖氧化损伤模型组(80 mg/kg D.Gal),阳性对照组(Vc,1 g/kg),鲜马奶粉低、中、高剂量组(40、80、120 mg/kg)。45 d后,测定小鼠肝脏、脾脏及血清中黄嘌呤氧化酶(XOD)、髓过氧化物酶(MPO)、蛋白质(考马斯亮蓝染色法)、一氧化氮合酶(NOS)、丙二醛(MDA)、谷胱甘肽(GSH)、一氧化氮(NO)、超氧物歧化酶(SOD)含量及观察小鼠体重变化趋势。结果显示:与模型组比较,鲜马奶粉可拮抗小鼠消瘦,增强血清、肝脏及脾脏中SOD和GSH的活性、减少MDA、MPO、XOD、NO、NOS的含量。鲜马奶粉具有阻止氧化损伤的作用,其机制可能与抑制自由基产生,增加体内抗氧化酶的活性,提高机体抗氧化能力有关。
To study the antioxidative effect of fresh horse milk powder on D-galactose-induced oxidative damage in mice. The mice were randomly divided into 6 groups: blank control group (normal saline), D-galactose oxidative damage model group (80 mg / kg D.Gal), positive control group (Vc, 1 g / kg) Milk powder low, medium and high dose groups (40,80,120 mg / kg). After 45 days, the levels of xanthine oxidase (XOD), myeloperoxidase (MPO), protein (Coomassie brilliant blue staining), nitric oxide synthase (NOS) (MDA), glutathione (GSH), nitric oxide (NO) and superoxide dismutase (SOD) in mice were observed. The results showed that compared with model group, fresh horse milk powder could antagonize the mice weight loss, enhance the activities of SOD and GSH in serum, liver and spleen and decrease the content of MDA, MPO, XOD, NO and NOS. Fresh horse milk powder has the function of preventing oxidative damage, and its mechanism may be related to inhibiting the production of free radicals, increasing the activity of antioxidant enzymes in the body and improving the antioxidant capacity of the body.