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目的 :通过检测牙科常用金合金及镍铬合金的细胞毒性及两种金属材料浸提液对小鼠成纤维细胞L929凋亡相关蛋白Bax和Bcl-2表达的影响,来研究金属离子与L929细胞凋亡的关系,探讨在分子水平上评价材料生物相容性的方法。方法:应用金合金(A组)及镍铬合金(B组)的浸提液培养小鼠成纤维细胞L929,以含10%胎牛血清的RPMI1640培养基做为阴性对照(C组),采用四甲基偶氮唑盐微量酶反应比色法(MTT法)评价2种金属材料的细胞毒性,采用免疫组化法及ELISA法(酶联免疫吸附实验)检测2种金属材料对L929细胞Bax和Bcl-2蛋白表达的影响。结果:2种材料的细胞毒性均为0级;镍铬合金组Bax/Bcl-2表达的比值高于阴性对照组及金合金组,差异有统计学意义。结论:镍铬合金浸提液可引起小鼠成纤维细胞L929凋亡相关蛋白Bax表达及Bax/Bcl-2比值增高,这一结果可能为将来在分子水平上建立评价生物材料生物相容性的方法提供新的思路。
OBJECTIVE: To study the effects of metal ions and L929 cells on the cytotoxicity of dental gold and nickel-chromium alloy and the effects of two metal leaching solutions on the expression of Bcl-2 and Bax in mouse fibroblast L929 fibroblasts Apoptosis of the relationship between the molecular level to evaluate the biocompatibility of materials. Methods: Mouse fibroblasts L929 were cultured with gold alloy (group A) and nickel-chromium alloy (group B). RPMI1640 medium containing 10% fetal bovine serum was used as a negative control (group C) The MTT method was used to evaluate the cytotoxicity of two metal materials. Immunohistochemistry and ELISA (enzyme-linked immunosorbent assay) were used to detect the effects of two metal materials on the Bax And Bcl-2 protein expression. Results: The cytotoxicity of the two materials were all grade 0. The ratio of Bax / Bcl-2 expression in the Ni-Cr alloy group was higher than that in the negative control group and the Au-alloy group. The difference was statistically significant. Conclusion: Ni-Cr alloy extract can induce apoptosis-related protein Bax expression and Bax / Bcl-2 ratio in mouse fibroblast L929 fibroblasts, and this result may be used to establish the molecular level to evaluate the biocompatibility of biomaterials Methods provide new ideas.