论文部分内容阅读
目的评价REXEEDTM系列透析器在高通量透析过程中白蛋白丢失特性。方法建立等容血液透析体外循环实验装置,采用REXEEDTM系列透析器进行高通量血液透析。实验根据透析器、血流量(Qb)、透析液流量(Qd)的不同组合进行分组(REXEEDTM-21AC,21UC,Qb=250 mL/min或350 mL/min,Qd=500 mL/min;REXEEDTM-15UC,Qb=250 mL/min,Qd=500 mL/min),每组透析重复3次,每次240 min。于实验前及实验开始后的不同时间点分别采集透析废液5 mL,酶联免疫法测定白蛋白浓度;根据曲线下面积计算每次透析过程丢失的白蛋白总量。结果REXEEDTM系列透析器在240 min的高通量透析过程中,白蛋白丢失总量<1.0 g;Qb=250 mL/min时,REXEEDTM-21AC和REXEEDTM-21UC透析白蛋白丢失量分别为(0.532±0.077)g和(0.219±0.097)g(P<0.05);当增加Qb时,白蛋白丢失有增加趋势,但无统计学意义。各组白蛋白丢失峰值出现在透析开始时至开始后10 min。结论REXEEDTM系列透析器在高通量透析过程中,白蛋白丢失量在临床可接受范围内。
Objective To evaluate the albumin loss characteristics of REXEEDTM dialyzer during high-throughput dialysis. Methods The establishment of isovolumic hemodialysis cardiopulmonary bypass experimental device, high-throughput hemodialysis using REXEEDTM series dialyzers. The experiments were grouped according to different combinations of dialyzer, blood flow (Qb) and dialysate flow (Qd) (REXEEDTM-21AC, 21UC, Qb = 250 mL / min or 350 mL / min, Qd = 500 mL / min; REXEEDTM- 15UC, Qb = 250 mL / min, Qd = 500 mL / min). Each dialysis was repeated 3 times for 240 min. Before the experiment and at different time points after the start of the experiment were collected dialysis waste 5 mL, enzyme-linked immunosorbent assay albumin concentration; according to the area under the curve calculate the total amount of albumin lost during each dialysis. Results The total amount of albumin loss in the REXEEDTM dialyzer during high-throughput dialysis at 240 min was less than 1.0 g. At Qb = 250 mL / min, the amounts of dialysis albumin lost to REXEEDTM-21AC and REXEEDTM-21UC were (0.532 ± 0.077) g and (0.219 ± 0.097) g, respectively (P <0.05). When Qb was increased, albumin loss tended to increase, but there was no statistical significance. Peak albumin loss in each group occurred at the beginning of dialysis and 10 min after the start of the dialysis. CONCLUSION REXEED ™ dialyzers have a clinically acceptable amount of albumin loss during high-throughput dialysis.