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本文报道应用Southern blot核酸分子杂交技术,以标记的国内HPV16型基因组分子为探针,共检测22例宫颈癌、4例宫颈不典型增生、6例宫颈慢性炎症,5例宫颈内膜息肉,6例正常宫颈上皮组织中的HPVl6型基因组同源序列。杂交结果表明,宫颈癌组织中HPV16型基因组同源序列阳性率为36.4％(相关序列阳性率为59.1％),非癌组织和正常宫颈上皮组织均为阴性,P<0.05具有显著性差异。同时还观察到宫颈癌病理组织学特征和组织细胞体外培养CPE与HPV16型基因组同源序列检出率呈正相关,P<0.001。更进一步证明宫颈癌发病与HPV感染密切相关。并提出HPV感染的初筛检测方法。
This paper reports the use of Southern blot hybridization of nucleic acid molecules to mark the domestic HPV16 genomic probe as a probe, a total of 22 cases of cervical cancer detection, 4 cases of cervical dysplasia, 6 cases of chronic cervical inflammation, 5 cases of cervical endometrial polyps, 6 cases of endometrial polyps, Example HPV16 genomic homology in normal cervical epithelium. The results of hybridization showed that the positive rate of HPV16 genomic homology in cervical cancer was 36.4% (the positive rate was 59.1%), while both non-cancerous tissue and normal cervical epithelium were negative, P <0.05 had significant difference. At the same time, the histopathological features of cervical cancer and the positive rate of detection of homologous sequences of HPV16 genotypes and CPE in tissue cells were also observed (P <0.001). Further prove that the incidence of cervical cancer and HPV infection are closely related. And proposed HPV infection screening test method.