论文部分内容阅读
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese su-peroxide dismutase expression in our tissue microarray was determined using immunohisto-chemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxoru-bicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 ex-pression was significantly negatively correlated with Bcl-2 and manganese superoxide dismu-tase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expres-sion produces a chemosensitivity in glioma.
Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) are an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese su-peroxide dismutase expression in our tissue microarray was determined using immunohisto- chemistry. , we constructed the LRIG1-U251 cell line, and its responses to doxoru-bicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 ex-pression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, ie in the LRIG1-U251 cell line, the chemosensitivity to doxoru bicin and temozolomide was increased. This indicates that increased LRIG1 expres-sion produces a chemosensitivity in glioma.