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随着喹诺酮类 (QNS)药物的广泛应用 ,QNS药物引起的不良反应也越来越引人们的重视 ,其中QNS类药物对儿童的软骨是否有损伤 ,是否会影响儿童的生长发育 ,至今还是一个争议较大的问题。在动物实验中 ,发现 QNS药物确实对幼龄动物的关节软骨引起损伤 ,主要见于负重关节 ,而且年龄越小损害程度越严重 ,并具有种属特异性。为确实 QNS药物对幼龄动物软骨的损伤、损伤的程度及损伤是否可逆 ,我们用微生物单层琼脂扩散法来监测环丙沙星 (CPL X)三个剂量组 [10 0 0、5 0 0、5 0 mg/ (kg· d) ]每天分 2次给药及空白组连续给药 10 d,停药后不同时间的膝关节的药物浓度及血中药物浓度 ,并对膝关节的软骨组织作了光、电镜观察。结果表明 ,环丙沙星对幼龄大鼠 (2 1d)的膝关节软骨有特异的亲和力 ,停药 2 4h后三个不同剂量组的软骨内的浓度均高于血药浓度 ,停药 6周后在膝软骨中还能测到药物浓度 ,三个不同剂量的膝软骨组织在光、电镜下均可见有明显的损伤 ,此病理损伤在停药 6周后还可见到。本实验再次表明 ,CPL X对幼龄动物软骨组织有明显的损伤作用。
With the widespread use of quinolones (QNS) drugs, adverse reactions caused by QNS drugs attract more and more attention. Among them, QNS drugs have no effect on the cartilage of children and will affect the children’s growth and development Controversial issues. In animal experiments, QNS drugs were found to cause damage to articular cartilage in young animals, mainly in weight-bearing joints, and the more severe the damage is, the more age-specific and species-specific. To confirm that cartilage damage, extent of injury and injury were reversible in QNS animals to young animals, we monitored the three doses of ciprofloxacin (CPL X) using the monolayer agar diffusion method [10 0 0 5 0 0 , 5 0 mg / (kg · d)] twice daily and the blank group for 10 days, the drug concentration in the knee at different time after drug withdrawal and blood drug concentration, and knee cartilage tissue Made a light, electron microscopy. The results showed that ciprofloxacin on young rats (21d) of knee cartilage has a specific affinity, withdrawal 24 h after three different doses of cartilage concentrations were higher than the plasma concentration, withdrawal 6 Weeks later in the knee cartilage can also be measured in drug concentrations, three different doses of knee cartilage tissue in light and electron microscopy can be seen obvious damage, the pathological injury after 6 weeks of withdrawal can be seen. This experiment again shows that, CPL X on young animals had significant damage to cartilage tissue.