论文部分内容阅读
目的:用去卵巢大鼠建立更年期动物模型,给予不同浓度孕酮,检测血清中一氧化氮(NO)含量的变化,观察孕酮对视网膜神经节细胞凋亡影响。方法:用放射免疫分析方法(RIA)测定血清中孕酮浓度,用比色法测定血清中NO的含量;用TUNEL原位凋亡技术检测视网膜神经节细胞凋亡。结果:NO浓度随孕酮水平增加而升高,视网膜神经节细胞凋亡与孕酮含量呈负相关。结论:适量孕酮可能通过促进NO生成,从而抑制视网膜神经节细胞的凋亡,进而发挥其视神经保护作用。
OBJECTIVE: To establish animal models of menopause in ovariectomized rats and to observe the changes of serum nitric oxide (NO) levels and the effects of progesterone on the apoptosis of retinal ganglion cells. Methods: The concentration of progesterone in serum was determined by radioimmunoassay (RIA), the content of NO in serum was determined by colorimetric method. The apoptosis of retinal ganglion cells was detected by TUNEL in situ apoptosis. Results: The concentration of NO increased with the increase of progesterone level. The apoptosis of retinal ganglion cells was negatively correlated with the content of progesterone. Conclusion: Moderate progesterone may inhibit the apoptosis of retinal ganglion cells by promoting the production of NO, and then exert its optic nerve protective effect.