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目的:研究XRCC1基因多态性与山东省人群胃癌易感性的关系。方法:采用1∶1病例-对照研究方法,收集山东籍原发性胃癌病例107例,同时随机选取同性别、同民族、年龄相差±5岁、同期住院的非肿瘤、非消化道系统疾病患者作为对照,采用PCR-RFLP方法分析XRCC1基因Arg194Trp、Arg280-His和Arg399Gln3个位点的多态性,比较不同基因型与胃癌易感性的关系。结果:在病例组和对照组194Trp的频率为53.27%和43.93%,280His的频率为12.15%和15.89%,399Gln的频率为54.21%和46.73%,差异均无统计学意义。在≥60岁年龄组携带194Trp等位基因的个体其胃癌风险增高(OR=2.303,95%CI=1.061～4.996,P=0.033),携带194Trp等位基因者可增加罹患胃底贲门癌的危险性(OR=2.766,95%CI=1.106～7.825,P=0.049)和发生远处转移的风险(OR=2.188,95%CI=1.022～4.688,P=0.042)。结论:XRCC1Arg194Trp基因多态性可增加老年人患胃癌的风险,与胃底贲门癌的发生有关,并促进胃癌的远处转移。Arg280His和Arg399Gln基因多态性与胃癌易感性无关联。
Objective: To study the relationship between XRCC1 gene polymorphism and gastric cancer susceptibility in Shandong province. Methods: One to one case-control study was conducted to collect 107 cases of primary gastric cancer in Shandong Province. At the same time, randomly selected patients of the same sex, same nationality, with a mean age of ± 5 years, hospitalized for non-tumor and non-gastrointestinal diseases As a control, polymorphisms of Arg194Trp, Arg280-His and Arg399Gln at XRCC1 were analyzed by PCR-RFLP and the relationship between different genotypes and gastric cancer susceptibility was compared. Results: The frequency of 194Trp was 53.27% and 43.93% in case group and control group. The frequency of 280His was 12.15% and 15.89%. The frequency of 399Gln was 54.21% and 46.73% respectively. There was no significant difference between the two groups. Individuals with the 194Trp allele in the age group of 60 years or older had an increased risk of gastric cancer (OR = 2.303, 95% CI = 1.061 to 4.996, P = 0.033). Patients with the 194Trp allele increased the risk of gastric cardia and cardia (OR = 2.766, 95% CI = 1.106-7.825, P = 0.049) and the risk of distant metastasis (OR = 2.188, 95% CI = 1.022-4.668, P = 0.042). Conclusion: The polymorphism of XRCC1Arg194Trp gene may increase the risk of gastric cancer in the elderly, which may be related to the occurrence of gastric cardia carcinoma and promote the distant metastasis of gastric cancer. There was no association between Arg280His and Arg399Gln polymorphisms and gastric cancer susceptibility.