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A series of novel 2,3-dihydro-2-phenylsulfonylhydrazono-3-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)thiazoles were designed and synthesized via the reaction of thiosemicarbazide with chloroaldehyde. Their chemical structures were characterized by 1H and 13C NMR spectroscopy and elemental analysis. The bioassay results indicate that some of these compounds exhibit moderate fungicidal and herbicidal activities.