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ＳＤ雄性大鼠侧脑室注射秋水仙素１００ｇ／１５Ｌ，２４ｈ后灌注固定，同时经侧脑室注射微量固定液加强对室管膜的固定。用免疫组化ＡＢＣ法研究Ｐ物质在ＳＤ雄性大鼠脑室管膜中的分布。结果显示，ＳＰ免疫阳性细胞分布于第三脑室侧壁、中脑导水管和延髓中央管的室管膜中，其中位于下丘脑结节区第三脑室侧壁及最后区平面延髓中央管的阳性室管膜细胞为伸长细胞，发出基突伸向周围灰质。本研究首次证实了含ＳＰ伸长细胞的存在，结合本实验室其他实验结果提示，这些伸长细胞在含肽的种类和区域分布上存在差异，为进一步深入研究伸长细胞在脑－脑脊液神经体液回路中的重要作用提供了化学神经解剖学依据。
SD male rats were injected intracerebroventricular injection of colchicines 100g / 15L, 24h after perfusion fixation, while the lateral ventricle injection of a small amount of fixative to strengthen the ependymal fixed. The distribution of substance P in the ependymal membrane of SD male rats was studied by immunohistochemical ABC method. The results showed that the SP immunopositive cells were located in the ependymal membrane of the lateral ventricle of the third ventricle, the midbrain aqueduct and the central canal of the medulla oblongata, and the posterior medulla oblongata central canal located in the lateral ventricle of the third ventricle in the hypothalamic nodular region and in the posterior region Ependymal cells for the elongation of cells, issued a base protruding to the surrounding gray matter. This study for the first time confirmed the existence of SP-containing cells, combined with other laboratory results suggest that these elongated cells in the peptide-containing species and regional distribution differences exist, in order to further study the elongation of cells in the brain - cerebrospinal fluid nerve An important role in the body fluid circuit provides a basis for chemical neuroanatomy.