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采用EST-SSR标记构建了国家种质广州甘薯圃中52份甘薯种质资源的DNA指纹图谱。利用52份供试资源,从早期筛选出的342对候选多态性引物中筛选出16对核心引物,16对引物在52份资源中共扩增出159个等位位点,每对引物的等位位点数为5~19个不等,平均为9.94个,多态性信息含量变化范围为0.6235~0.9593,平均为0.7991。选择带型清晰、重复性好、易于统计且能将52份资源完全区分开的2对引物组合构建供试资源的DNA指纹图谱。NTSYS软件聚类分析表明,EST-SSR标记能正确反映出不同资源间的亲缘关系,为构建甘薯大型DNA指纹图谱数据库奠定了基础。
The DNA fingerprinting of 52 sweet potato germplasm resources in Guangzhou sweet potato nursery was constructed using EST-SSR marker. Fifty-two pairs of candidate polymorphic primers were screened out from 52 early-selection primers using 16 accessions. Fifteen alleles were amplified by 16 pairs of primers from 159 pairs of primers in 52 accessions. The median loci ranged from 5 to 19, with an average of 9.94. The polymorphism information content ranged from 0.6235 to 0.9593 with an average of 0.7991. A DNA fingerprinting was constructed using two pairs of primers with clear bands, good repeatability, easy statistics and complete separation of 52 resources. NTSYS software clustering analysis showed that EST-SSR markers can correctly reflect the genetic relationship between different resources, which laid the foundation for constructing large-scale DNA fingerprinting database of sweet potato.