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探讨臭氧作为土壤熏蒸剂防控蔬菜土传真菌病害的理论依据。研究离体条件下臭氧熏蒸处理对黄瓜枯萎病菌、立枯丝核菌和甘蓝枯萎病菌3种土传病原真菌菌丝生长和孢子萌发的抑制作用。结果表明,臭氧质量浓度为62～72mg/m3处理对3种供试病菌菌丝生长显著抑制,随处理时间延长,抑制率提高,当达到120min时,抑制率均达到了90%以上均显著高于30和60min的处理,并且菌丝量明显减少,菌丝体发生聚集、卷曲、局部膨大,菌丝隔膜消失、原生质体溶解。黄瓜枯萎病菌和甘蓝枯萎病菌的孢子在水琼脂上涂板,臭氧处理质量浓度为40mg/m3,总体表现为病菌孢子涂板后培养时间越短,臭氧处理时间越长,臭氧对病菌孢子的萌发抑制率越高:病菌孢子涂板后立即处理3min,对孢子的萌发抑制率达到100%,而涂板后培养10h,处理1min,对孢子萌发的抑制作用不明显。臭氧质量浓度为40mg/m3处理3min对孢子具有杀灭作用,相同条件下对菌丝生长的抑制作用较小,说明了孢子对臭氧比菌丝敏感。臭氧具有抑制多种土传病原真菌的良好效果,尝试臭氧作为土壤熏蒸剂防治蔬菜生产中土传真菌病害的发生和危害值得进一步探索。
To explore the theoretical basis of ozone as soil fumigant to prevent and control vegetable soil fungus disease. To study the inhibitory effect of ozone fumigation on mycelial growth and spore germination of Fusarium oxysporum, Rhizoctonia solani and Fusarium oxysporum f. The results showed that the ozone concentration of 62 ~ 72mg / m3 could significantly inhibit the growth of mycelia of three tested pathogens. With the prolongation of treatment time, the inhibition rate increased. When reaching 120min, the inhibition rate reached more than 90% At 30 and 60 min treatment, the amount of mycelia decreased significantly, mycelium aggregated, curled, partially enlarged, hypha membrane disappeared and protoplast was dissolved. Cucumber Fusarium oxysporum and Fusarium oxysporum spores were coated on water agar, the mass concentration of ozone treatment was 40mg / m3, the overall performance of the spores of bacteria after the incubation time is shorter, the longer the ozone treatment, ozone spores germ The higher the inhibition rate, the spores of the bacteria were treated immediately after being coated for 3 minutes, and the inhibition rate to spore germination was 100%. After being coated for 10 hours and treated for 1 minute, the inhibition effect on spore germination was insignificant. Ozone concentration of 40mg / m3 treatment 3min on the spores have a killing effect under the same conditions on mycelial growth inhibition is small, indicating that the spores of ozone than the mycelium sensitive. Ozone has a good effect of inhibiting a variety of soil-borne pathogenic fungi, try ozone as a soil fumigant prevention and control of soil-borne fungus in the production of vegetable diseases and hazards worth further exploration.