论文部分内容阅读
目的研究半胱氨酸天冬氨酸特异性蛋白酶-3(Caspase-3)在大肠肿瘤组织中的表达情况及其与大肠癌临床病理特征的关系,从而评价其在大肠癌发生发展中的作用。方法采用免疫组织化学方法分别检测Caspase-3在66例大肠癌、23例腺瘤性息肉及10例正常结肠黏膜组织中的表达情况及其与大肠癌临床病理特征的关系,采用SPSS 11.0对数据进行统计学分析。率的比较采用χ2检验,P<0.05为差异有统计学意义。结果 Caspase-3在大肠癌、大肠腺瘤性息肉及正常结肠黏膜组织中的阳性表达率分别为75.8%、56.5%和70.0%,Caspase-3在大肠癌中阳性表达率明显高于息肉组(P<0.05),而在大肠癌组与正常组之间、息肉组与正常组之间差异无统计学意义;Caspase-3表达与患者性别有关(P<0.05),而与患者年龄、肿瘤大小、分布部位、分化程度、有无淋巴结转移、有无远处转移及TNM分期等临床病理特征无关。结论腺瘤性息肉组织中Caspase-3呈低表达现象,提示Caspase-3低表达在大肠癌发生早期可能起一定作用。
Objective To investigate the expression of caspase-3 in colorectal carcinoma and its relationship with the clinicopathological features of colorectal cancer so as to evaluate the role of Caspase-3 in the development of colorectal carcinoma . Methods The expression of Caspase-3 in 66 cases of colorectal carcinoma, 23 cases of adenomatous polyps and 10 cases of normal colorectal mucosa were detected by immunohistochemistry. The relationship between the expression of Caspase-3 and the clinicopathological features of colorectal cancer was analyzed by SPSS 11.0 Statistical analysis. Rate comparison using χ2 test, P <0.05 for the difference was statistically significant. Results The positive rate of Caspase-3 expression in colorectal carcinoma, adenomatous polyps and normal colorectal mucosa was 75.8%, 56.5% and 70.0% respectively. The positive rate of Caspase-3 in colorectal carcinoma was significantly higher than that in polyp P <0.05), but there was no significant difference between the colorectal cancer group and the normal group (P <0.05). The expression of Caspase-3 was correlated with gender (P <0.05), but not with age, tumor size , Distribution location, degree of differentiation, lymph node metastasis, distant metastasis and TNM staging and other clinical pathological features. Conclusions The low expression of Caspase-3 in adenomatous polyps suggests that the low expression of Caspase-3 might play an important role in the early stage of colorectal cancer.