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目的探讨使用低剂量环磷酰胺对血吸虫感染免疫的影响。方法小鼠经腹腔注射低剂量环磷酰胺(50mg/kg),在体内创造并维持一个Th1型免疫环境。观察小鼠外周血、脾脏调节性T细胞水平及血清细胞因子的动态变化。采用半定量逆转录聚合酶链反应检测经环磷酰胺处理的调节性T细胞上Foxp3基因表达水平。用(30±1)条血吸虫尾蚴感染处于Th1型免疫反应状态小鼠,42d后剖杀,计算减虫率、减卵率,通过组织切片观察小鼠肝脏组织病理变化。结果低剂量环磷酰胺可以降低小鼠外周血、脾脏中的调节性T细胞水平,并下调Foxp3的表达,使小鼠血清IL-2、IFN-γ上升,IL-4、IL-10及TGF-β水平下降,免疫反应向Th1型转变。与对照组相比,用药组的血吸虫虫卵产量明显下降,小鼠肝脏虫卵肉芽肿病变明显减轻。结论低剂量环磷酰胺可调节小鼠的免疫反应朝Th1发展,可以降低血吸虫虫卵产量,并减轻肝脏虫卵肉芽肿病变。
Objective To investigate the effect of low dose cyclophosphamide on schistosoma infection. Methods Mice were injected intraperitoneally with low dose cyclophosphamide (50mg / kg) to create and maintain a Th1 immune environment in vivo. The changes of peripheral blood and spleen Tregs and serum cytokines in mice were observed. Semi-quantitative reverse transcriptase-polymerase chain reaction was used to detect Foxp3 gene expression on cyclophosphamide-treated regulatory T cells. (30 ± 1) cercariae Schistosome infection in Th1 type immune response mice, 42d after the killing, calculate the worm reduction rate, the rate of egg reduction, observed by histological sections of mice liver pathological changes. Results Low dose cyclophosphamide can reduce the level of regulatory T cells in peripheral blood and spleen of mice and down-regulate the expression of Foxp3, increase the levels of IL-2, IFN-γ, IL-4, IL- -β level decreased, the immune response to Th1-type transition. Compared with the control group, the schistosomiasis egg production of the treated group was significantly decreased, and the lesions of the mouse hepatic egg granuloma significantly reduced. Conclusion Low dose cyclophosphamide can modulate the immune response of mice toward Th1, which can reduce the egg production of Schistosoma japonicum and reduce the incidence of hepatic egg granulomatous lesions.