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为了解青州市肾综合征出血热(HFRS)的感染类型,探讨其影响因素,于1996年进行了本次调查,结果报告如下:1 材料与方法1.1 传染源调查 将用夹夜法捕获的鼠只解剖取鼠肺,用间接免疫荧光法(IFAT)检测HFRS抗原.以当地病人阳性血清为第一抗体,上海生物制品研究所生产的羊抗人IgG抗体(批号9503)为第二抗体,伊文思染色,滴度≥1:20为阳性.
In order to understand the type of infection of Hemorrhagic Fever with Renal Hemorrhagic Fever (HFRS) in Qingzhou and to explore its influencing factors, this survey was conducted in 1996 and the results are reported as follows: 1 Materials and Methods 1.1 Infection Source Investigation Murine Only the rat lungs were dissected and HFRS antigen was detected by indirect immunofluorescence assay (IFAT) .The positive antibody of local patients was used as the first antibody, goat anti-human IgG antibody (batch No. 9503) produced by Shanghai Institute of Biological Products as the second antibody, Evans staining, titer ≥1: 20 is positive.