论文部分内容阅读
目的:探讨血浆纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor-1,PAI-1)基因多态性与2型糖尿病肾病(diabetic nephropathy,DN)的相关性,并初步研究其可能机制。方法:50例正常对照组,120例2型糖尿病,测定任意尿白蛋白与肌酐比值(Ua/c),根据比值将糖尿病组分为50例糖尿病非肾病组、70例糖尿病肾病组。提取DNA,聚合酶链反应扩增,产物以琼脂糖凝胶电泳分离确定PAI-1基因型。并收集体重、身高、血压、血糖、糖化血红蛋白、甘油三酯、胆固醇等资料。结果:(1)正常人、糖尿病非肾病与糖尿病肾病组相比,PAI-14G/4G基因型的分布有明显的差别,糖尿病肾病组PAI-1基因中4G等位基因频率明显高于糖尿病非肾病组(0.550∶0.149,χ2=35.685,P<0.05)。(2)Logistic回归分析表明PAI-1基因是2型糖尿病肾病的独立患病危险因素。PAI-14G/4G基因型比其他基因型具有更高的糖尿病肾病发病危险。结论:2型糖尿病肾病组PAI-1基因4G/4G型和4G等位基因频率明显高于正常人、2型糖尿病非肾病组。PAI-1基因4G/4G型是2型糖尿病肾病发病的独立患病危险因素。PAI-14G/4G型为糖尿病肾病发病的易感性基因。
Objective: To investigate the correlation between plasma PAI-1 gene polymorphism and type 2 diabetic nephropathy (DN), and to investigate its possible mechanism . Methods: Fifty normal controls and 120 cases of type 2 diabetes mellitus were enrolled in this study. The ratio of urinary albumin to creatinine (Ua / c) was determined. According to the ratio, the diabetic patients were divided into 50 diabetic nephropathy patients and 70 diabetic nephropathy patients. DNA was extracted and amplified by polymerase chain reaction (PCR). The PAI-1 genotypes were separated by agarose gel electrophoresis. And collected weight, height, blood pressure, blood glucose, glycosylated hemoglobin, triglycerides, cholesterol and other data. Results: (1) The distribution of PAI-14G / 4G genotypes were significantly different in normal subjects, diabetic nephropathy patients and diabetic nephropathy patients. The frequency of 4G allele in PAI-1 gene was significantly higher in diabetic nephropathy patients than in diabetic nephropathy patients Nephropathy group (0.550: 0.149, χ2 = 35.685, P <0.05). (2) Logistic regression analysis showed that PAI-1 gene is an independent risk factor for type 2 diabetic nephropathy. The PAI-14G / 4G genotype has a higher risk of diabetic nephropathy than other genotypes. Conclusion: The frequencies of 4G / 4G and 4G alleles of PAI-1 gene in type 2 diabetic nephropathy group were significantly higher than those in normal people and type 2 diabetic nephropathy group. PAI-1 gene 4G / 4G is an independent risk factor for type 2 diabetic nephropathy. PAI-14G / 4G type is a susceptibility gene for the pathogenesis of diabetic nephropathy.