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目的:通过观察4周2500米高住低训过程中,人体红细胞CD35数量和活性变化,探讨灵芝多糖对高住低训中人体红细CD35的影响。方法:以16名北京体育大学体育教育学院足球专项运动员为受试对象,随机分为吃药组和对照组各8名,均为高住低训。两组每晚入住低氧房(O2浓度15.4%,相当于海拔2500米)10小时,每周2次低氧房72%最大摄氧量蹬功率自行车30分钟训练,并且两组每周有3次同一教练执导的专项训练。取吃药前,暴露前、入住10小时、入住2周、3周、4周时清晨静脉血,与相应的荧光标记抗体反应,用流式细胞仪记录其平均荧光强度、阳性细胞率。结果:4周实验后,吃药组和对照组红细胞CD35的表达较实验前分别升高了7.9%和下降了12.8%(P<0.05),吃药组和对照组红细胞C3b受体花环率较吃药前分别升高了45.9%(P<0.05)和下降了49.0%(P<0.05),两组相比有显著性差异,吃药组和对照组红细胞IC花环率较实验前分别升高了99.7%(P<0.01)和19.5%。结论:灵芝多糖可以明显影响红细胞CD35数量的表达,并且可以调节高住低训实验中出现的运动员红细胞继发性免疫低下的现象。
Objective: To observe the effect of Ganoderma lucidum polysaccharides on human erythrocyte CD35 in high school and low lab training by observing the change of the number and activity of human erythrocyte CD35 during the 2500-meter-high school training in 4 weeks. Methods: Sixteen athletes from Beijing Sport University Physical Education College were enrolled in this study. They were randomly divided into two groups: eight in each group, and eight in control group. The two groups were admitted to hypoxia room (O2 concentration 15.4%, equivalent to 2500 meters above sea level) for 10 hours every night, hypoxia room 72% maximum oxygen uptake per week for 30 minutes and power cycling for 3 minutes The same coach directed the special training. Before medication, before exposure, stay 10 hours, stay 2 weeks, 3 weeks, 4 weeks early morning venous blood, and the corresponding fluorescent labeled antibody reaction, the average fluorescence intensity was recorded by flow cytometry, the positive cell rate. Results: After 4 weeks, the expression of CD35 in erythrocytes in the drug-treated group and the control group increased by 7.9% and 12.8%, respectively (P <0.05), compared with those in the control group Before taking medicine, they increased 45.9% (P <0.05) and decreased 49.0% (P <0.05) respectively. There was a significant difference between the two groups. The IC rosette rate of erythrocytes in the medication group and the control group increased respectively 99.7% (P <0.01) and 19.5%. Conclusion: Ganoderma lucidum polysaccharides can significantly affect the expression of erythrocyte CD35, and can modulate the phenomenon of secondary immune compromise of red blood cells in athletes in high and low training.