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Objective:To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii(P.carina) against P.carina in mice.Methods:The fragment of the antigen within p55(p55-582) was cloned.Then recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+).BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-p55-582.ELBA and RTPCR were used to evaluate the role of this kind of DNA vaccine.Results:The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582]could be expressed correctly and had antigenicity in transfected COS-7 cells.ELBA and RT-PCR showed that pcDNA3.1(+)- p55-582 elicited antibody production,stimulated lymphocyte proliferation and provided partial protection by reducing the P.carina burden.Conclusions:The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.
Objective: To evaluate the protective effect of DNA vaccine with the gene encoding 55 kDa antigen fragment of Pneumocystis carinii (P. Carina) against P. carina in mice. Methods: The fragment of the antigen within p55 (p55-582) was cloned.Then The recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1 (+). BALB / c mice were used as experimental models to examine the immunogenicity of pcDNA3.1 (+) - p55-582. ELBA and RTPCR were used to evaluate the role of this kind of DNA vaccine. Results: The results of western blot indicated that the recombinant DNA [pcDNA3.1 (+) - p55-582] could be expressed correctly and had antigenicity in transfected COS-7 cells. PCR showed that pcDNA3.1 (+) - p55-582 elicited antibody production, stimulated lymphocyte proliferation and provided partial protection by reducing the P.carina burden. Conclusions: The data demonstrate that pcDNA3.1 (+) - p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.