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目的 明确精子结合抗原11C(SPAG11C)和11T(SPAG11T)在大鼠附睾中的表达定位,探究实验性左侧精索静脉曲张(ELV)对其表达的影响,为研究精索静脉曲张致不育机制提供实验资料.方法 48只成年SD大鼠随机分为4组:假手术2周组(A组)、假手术4周组(B组)和ELV造模2周组(C组)、ELV造模4周组(D组),每组12只.造模组行左肾静脉部分结扎术,建立实验性左侧精索静脉曲张模型;假手术组仅暴露左肾静脉.采用实时定量聚合酶链反应和免疫组织化学的方法,检测SPAG11C和SPAG11T在各组双侧附睾组织中的表达定位及变化.结果 SPAG11C和SPAG11T主要表达在附睾上皮的主细胞胞质内,且其表达呈现区段特异性.在ELV造模2周和4周后,造模组大鼠左侧附睾中SPAG11C和SPAG11T在核酸和蛋白水平上的表达均较同侧假手术组及造模组右侧附睾显著降低(P<0.05),且与ELV造模2周组相比,ELV造模4周组降低更为明显(P0.05).结论 SPAG11C和SPAG11T在附睾中的表达具有细胞特异性和区段特异性,且精索静脉曲张可致其表达显著降低,可能是精索静脉曲张导致附睾微环境改变,影响精子成熟,继而导致男性生育力降低的原因之一.“,”Objective To investigate the expression and distribution of sperm associated antigen 11C and T (SPAG11C and T) in the epididymis of adolescent rats with experimental left varicocele (ELV) model, and to explore the mechanism of varicocele-induced subfertility. Methods The partial ligation of left renal vein was adopted to establish ELV rat model, parallel with the sham treatment meanwhile. Total of 48 SD rats were divided into four groups: ELV-2 weeks and 4 weeks groups, sham-2 weeks and 4 weeks groups. The expressions of SPAG11C and T were detected by the real-time PCR and immunohistochemistry. Results SPAG11C and T were mainly distributed in the cytoplasm of principle cells of the epididymal epithelium. The immunostaining density of SPAG11C was strong in the proximal corpus, and weak gradually from the middle corpus to the cauda. The strong expression of SPAG11T was detected in the proximal corpus and the cauda, but the weak signal existed in the distal corpus. ELV-2 and 4 weeks later, the expressions of SPAG11C and T stayed normal level in the bilateral epididymides of sham groups and in the right side of ELV groups. Whereas the significant decreases of expressions of SPAG11C and T were found in the left epididymides of ELV groups, compared with sham operation group and ELV right epididymides. The heavier slump was found in the ELV left side at 4 weeks. Conclusion SPAG11C and T are specific proteins expressed in the epididymis, and they involved in the composition of epididymal microenvironment. ELV downregulated their expression levels significantly. Decrease of them may attribute to varicocele-caused male subfertility.