论文部分内容阅读
目的了解云南傣族3个STR位点遗传多态性分布。方法应用多重PCR技术对HUMCSF1PO、HUMTPOX和HUMTH013个STR基因座进行分析,采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术,对云南傣族上述3个基因座进行遗传多态性调查。结果CSF1PO位点,观察到7个等位基因,15个基因型;TPOX位点,观察到7个等位基因,18个基因型;TH01位点,观察到6个等位基因,17个基因型。结论上述3个短串联重复序列基因座基因频率分布与Hardy-Weiberg平衡吻合良好。
Objective To understand the genetic polymorphism distribution of three STR loci in Yunnan Dai nationality. Methods Multiplex PCR (PCR) was used to analyze the STR loci of HUMCSF1PO, HUMTPOX and HUMTH013. High-resolution polyacrylamide gel electrophoresis and silver staining were used to detect the genetic polymorphism of these three loci in Yunnan province. survey. Results There were 7 alleles and 15 genotypes in the CSF1PO locus. Seven TP alleles and 18 genotypes were observed in the TPOX locus. Th01 alleles, 6 alleles and 17 genes were observed type. Conclusion The frequency distribution of the three short tandem repeat loci was in good agreement with Hardy-Weiberg equilibrium.