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目的:探讨Bax基因对肝癌细胞促凋亡作用及转基因途径对转染效率的影响。方法:双腺病毒载体系统介导Bax基因转染人肝癌细胞系QGY7703,并将Bax基因通过鼠尾静脉、肝动脉进行转染,观察细胞凋亡及不同转染途径对靶器官的影响。结果:1)Bax基因转染导致人肝癌细胞凋亡。实验组凋亡百分率明显高于各对照组。2)肝动脉插管组LacZ在靶肝细胞转染率高;非靶器官分布表达少,靶/非靶比值大(P<0.05);阻断血供有利于以较小病毒滴度获得较高的转染率;尾静脉组靶肝细胞转染率低,在非靶器官分布表达多。结论:应用肝动脉超选择技术,经肝动脉传输Bax基因治疗肝癌具有安全、低毒和靶向选择性,为Bax经肝动脉基因治疗提供了实验依据。
Objective: To investigate the effect of Bax gene on apoptosis of hepatocellular carcinoma cells and the effect of transgene on transfection efficiency. Methods: Bax gene was transfected into human hepatocarcinoma cell line QGY7703 by double adenovirus vector system. Bax gene was transfected through the tail vein and hepatic artery of rats to observe the effects of apoptosis and different transfection routes on target organs. Results: 1) Bax gene transfection led to apoptosis of human hepatoma cells. The apoptosis rate of experimental group was significantly higher than that of control group. 2) The transfection rate of LacZ in target hepatic artery in hepatic arterial cannulation group was high; the expression of non-target organs was low and the target / non-target ratio was high (P <0.05); High transfection efficiency; low transfection efficiency of target hepatocytes in the tail vein group, more expression in non-target organs. CONCLUSIONS: Transhepatic artery delivery of Bax gene to hepatocellular carcinoma is safe, low toxic and targeted selective. It provides an experimental basis for gene therapy of Bax via hepatic artery.