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目的 研究肝门部血流阻断后肠粘膜损害所致细菌移位及门静脉血中内毒素的变化。方法 采用兔动物模型,在无菌条件下机械性阻断肝十二指肠韧带,观察阻断后15,20,30min和60min时门静脉血中内毒素值和肠系膜淋巴结细菌培养阳性数。结果 实验组肝门阻断后门静脉血内毒素值和细菌培养阳性数明显高于对照组(P<0.01),且随阻断时间延长加重。结论 缺血、缺氧是肠粘膜屏障损害的直接原因,深入对肠粘膜屏障功能保护的研究将有利于指导临床工作。
Objective To study the bacterial translocation caused by intestinal mucosal lesion and the change of endotoxin in portal vein after hepatic portal blood flow occlusion. Methods Rabbit model was used to mechanically block the hepatoduodenal ligaments under aseptic conditions. The numbers of endotoxin in portal vein blood and the number of positive bacterial culture in mesenteric lymph nodes were observed at 15, 20, 30 and 60 minutes after the occlusion. Results In the experimental group, the values of portal vein blood endotoxin and bacterial culture positive number after hepatic portal vein occlusion were significantly higher than those of the control group (P <0.01), and increased with the prolongation of the blocking time. Conclusion Ischemia and hypoxia are the direct causes of intestinal mucosal barrier damage, and further studies on the protective function of intestinal mucosal barrier will be helpful to guide the clinical work.