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目的:优选岗松总黄酮的大孔吸附树脂纯化工艺。方法:以总黄酮吸附率和洗脱率为指标,采用UV测定总黄酮含量,筛选最佳大孔吸附树脂,并通过单因素试验考察岗松总黄酮的大孔树脂纯化工艺。结果:采用AB-8型大孔吸附树脂对岗松总黄酮进行纯化,其最佳工艺条件为上样液生药质量浓度0.3 g.mL-1,上样流速1 BV.h-1,上样体积3 BV,加50%乙醇5BV以2 BV.h-1的流速洗脱,总黄酮纯度达52.33%。结论:优选的工艺合理可行,适用于工业化生产。
Objective: To optimize the macroporous adsorption resin purification technology of total flavonoids from Gang pine. Methods: The total flavonoids adsorption rate and elution rate as an indicator, the total flavonoid content was determined by UV, the best macroporous resin was screened, and the macroporous resin purification technology of the total flavonoids of Pinus sylvestris was investigated by single factor test. Results: The total flavonoids were purified by AB-8 macroporous resin. The optimum conditions were as follows: the mass concentration of crude drug was 0.3 g.mL-1, the flow rate was 1 BV.h-1, The volume of 3 BV, with 50% ethanol 5BV at a flow rate of 2 BV.h-1, the total flavonoids purity of 52.33%. Conclusion: The preferred process is reasonable and feasible and suitable for industrial production.