皮肤型人乳头瘤病毒DNA在皮肤肿瘤表面高检出,而在活检标本中检出率低

• 作 者: Forslund O. Lindelf B.adil E. 崔荣
• 机 构: Department of Medical Microbiology, Malmo University Hospital, SE-205 02 Malmo, Sweden
• 出 处: 世界核心医学期刊文摘(皮肤病学分册)
• 发表日期: 2005年01期
• 关键词: 皮肤肿瘤 活检标本 人乳头瘤病毒 检出 皮肤型 拭子 脂溢性角化病 基底细胞癌 角化症 光化性

Genomes of human papillomaviruses (HPV) are common in biopsies from non-melanoma skin cancers but are also found on healthy skin and it is possible that HPV positivity in tumor biopsies by PCR maymerely reflect contamination of the lesion surface.To investigate this issue, 229 immunocompetent patients were tested for HPV DNA in swab samples collected on top of skin tumors and in biopsies of the same tumors, obtained after stripping with tape to remove superficial layers.HPV DNA was detected on top of 69%(159 of 229) of the lesions, and in 12%(28 of 229) of the stripped biopsies (p < 0.001).The difference was seen for all four types of tumors studied.Seborrheic keratosis had 79%(34 of 43) HPV positivity on top of lesions versus 19%(eight of 43) in biopsies; actinic keratosis had 83%(38 of 46) HPV positivity on top versus 11%(five of 46) in biopsies; basal cell carcinoma had 63%(69 of 109) on top versus 8%(nine of 109) in biopsies and squamous cell carcinoma had 58%(18 of 31) on top versus 19%(six of 31) in biopsies.HPV DNA is common in superficial layers of lesions, but is not necessarily present throughout tumors.

Genomes of human papillomaviruses (HPV) are common in biopsies from non-melanoma skin cancers but are also found on healthy skin and it is possible that HPV positivity in tumor biopsies by PCR maymerely reflect contamination of the lesion surface. To investigate this issue, 229 immunocompetent patients were tested for HPV DNA in swab samples collected on top of skin tumors and in biopsies of the same tumors, obtained after stripping with tape to remove superficial layers. HPV DNA was detected on top of 69% (159 of 229) of the lesions of, and in 12% (28 of 229) of the stripped biopsies (p <0.001) .The difference was seen for all four types of tumors studied. Seborrheic keratosis had 79% (34 of 43) HPV positivity on top of lesions versus (8 of 43) in biopsies; actinic keratosis had 83% (38 of 46) HPV positivity on top versus 11% (five of 46) in biopsies; basal cell carcinoma had 63% (69 of 109) on top versus 8 % (nine of 109) in biopsies and squamous cell carcinoma had 58% (18 of 31) on top vers us 19% (six of 31) in biopsies. HPV DNA is common in superficial layers of lesions, but is not necessarily present throughout tumors.