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目的 研究细菌L型感染与子宫内膜腺癌的相关性。方法 用革兰染色、免疫组化和DNA核酸探针原位杂交等方法 ,对 6 6例子宫内膜腺癌 ,30例子宫内膜增生症进行检测。结果 子宫内膜腺癌和内膜增生症中革兰氏染色细菌L型阳性率分别为 5 4 5 % ,5 6 7% ;免疫组化染色 (S -P法 )检测阳性率分别为 5 6 1%和 6 0 6 % ;2 0例子宫内膜腺癌标本 ,用金黄色葡萄球菌 (金葡菌 )L型地高辛标记的核酸探针作原位杂交 ,结果发现 45 %的宫内膜腺上皮细胞核内显示有金葡菌L型DNA阳性颗粒。结论 提示细菌及其L型可侵入子宫内膜腺上皮细胞 ,导致细胞异常增生甚至肿瘤发生。证明子宫内膜腺癌的发生与细菌L型感染有一定的相关性。
Objective To study the correlation between bacterial L-type infection and endometrial adenocarcinoma. Methods Sixty-six cases of endometrial adenocarcinoma and 30 cases of endometrial hyperplasia were examined by Gram stain, immunohistochemistry and in situ DNA probe hybridization. Results The positive rates of gram positive bacteria in endometrial adenocarcinoma and intimal hyperplasia were 54.5% and 56.7%, respectively. The positive rates of immunohistochemical staining (S-P method) were 56 1%, and 60.6%; 20 cases of endometrial adenocarcinoma specimens, Staphylococcus aureus (Staphylococcus aureus) L-digoxin labeled nucleic acid probe for in situ hybridization and found that 45% of intrauterine Membranous glandular epithelial cells showed S. aureus L-type DNA positive particles. Conclusions suggest that bacteria and their L-type can invade the endometrial glandular epithelial cells, leading to abnormal cell proliferation and even tumorigenesis. Proves the occurrence of endometrial adenocarcinoma and bacterial L-type infection have some relevance.