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The chromatin folding patterns in air and liquid were studied by atomicforce microscopy (AFM). A gentle water-air interface method was adopted to spreadchromatin from interphase nucleus of chicken erythrocyte. The chromatin was ah-sorbed on APS-Inica surfaCe and studied with AFM. Beads-on-a-string were observedand many higher-order structures such as superbeads with dimensions 40-60urn indiameter and 4-7 urn in height were found to string together to make chromatin fibers.When sample spreading and absorbing time were shortened, higher-order chromatinfibers with 60-120 urn in width were observed in air as well as under water environment. These chromatin structures may reflect chromatin folding patterns in the livingcells.
The chromatin folding patterns in air and liquid were studied by atomic force microscopy (AFM). A gentle water-air interface method was adopted to spread chromatin from interphase nucleus of chicken erythrocyte. The chromatin was ah-sorbed on APS-Inica surfaCe and studied with AFM Beads-on-a-string were observed and many higher-order structures such as superbeads with dimensions 40-60urn indiameter and 4-7 urn in height were found to string together to make chromatin fibers. WHEN sample spreading and absorbing time were shortened, higher-order chromatinfibers with 60-120 urn in width were observed in air as well as under water environment. These chromatin structures may reflect chromatin folding patterns in the living cells.