A Mew Technique to Rapidly Test Agrochemicai Residues In Fruits and Vegetables

• 出 处: HuNan Agricultural Science & Technology Newsletter
• 发表日期: 2000年02期
• 关键词: AchE DTNB vegetables hydrolysis decided residues saline distilled Enzyme pestici

Extraction of AchE, relationship between substrate and enzyme concentration, and inhibition effects of the agrochemicals to AchE are discussed in this paper. Through the re-search, the proper AchE concentration for hydrolysis of 1 ml 1mmol/L substrate and I50 val-ues of the agrochemicals to AchE are decided. It is proved that Asch-DTNB method is a rapid test tool for agrochemical residues in fruits and vegetables. A rapid test card has been developed with sensitivity of 0.05mg/L.

Extraction of AchE, relationship between substrate and enzyme concentration, and inhibition effects of the agrochemicals to AchE are discussed in this paper. Through the re-search, the proper AchE concentration for hydrolysis of 1 ml 1 mmol / L substrate and I50 val-ues of The agrochemicals to AchE are decided. It is proved that Asch-DTNB method is a rapid test tool for agrochemical residues in fruits and vegetables. A rapid test card has been developed with sensitivity of 0.05 mg / L.