T-AK细胞的细胞毒活性研究

• 作 者: 魏虎来 马海珍 赵怀顺
• 机 构: 兰州医学院血液病研究所
• 出 处: 中国免疫学杂志
• 发表日期: 1999年03期
• 关键词: T-AK细胞 细胞毒活性 异质性

目的：探讨抗ＣＤ３单抗（ＣＤ３ＭｃＡｂ）和ｒＩＬ２共同激活诱生的ＴＡＫ（Ｔａｃｔｉｖａｔｅｄｋｉｌｅｒ）细胞的细胞毒活性本质。方法：采用ＭＴＴ法分别检测ＴＡＫ细胞杀伤白血病细胞的活性及其构成。结果：ＴＡＫ细胞杀伤白血病细胞的活性主要由４部分组成：ＣＤ３ＭｃＡｂ激活的ＣＤ３ＡＫ活性约占５０％，ｒＩＬ２诱生的ＬＡＫ细胞活性约为３０％，ＮＫ活性约占１０％，可溶性抑制性因子的抑制活性约为１０％；细胞表型分析ＴＡＫ细胞主要表达活化Ｔ淋巴细胞和ＮＫ细胞的表面标志。结论：ＴＡＫ细胞为异质性细胞群体，其杀伤活性主体为ＣＤ３ＡＫ活性和ＬＡＫ活性

Objective: To investigate the cytotoxic activity of TAK (Tactivated killer) cells induced by CD3McAb and rIL2 co-activation. Methods: MTT assay were used to detect TAK cell killing leukemia cell activity and its composition. RESULTS: The activity of T-AK cell-killing leukemia cells was mainly composed of four parts: about 50% of CD3AKs activated by CD3McAb, about 30% of LAK cells induced by rIL-2, about 10% of NK activity, and soluble inhibition The inhibitory activity of the factor is about 10%; Cell phenotype analysis T-AK cells mainly express the surface markers of activated T lymphocytes and NK cells. Conclusion: T  AK cells are heterogeneous cell populations, the main killer activity of CD3AK activity and LAK activity