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从许多文献上看,有关蜜蜂幼虫体内微生物的研究报道是相互抵触的。这可能是由于研究方法(如幼虫匀浆及幼虫肠道匀浆;组织学及微生物学的技术;微生物培养基的选择;以及幼虫匀浆过程中稀释液的使用而导致幼虫肠道中少量微生物分离机会的减少)的缘故。所以再次设计了对于分离少量微生物效果最好的方法以研究幼虫肠道的微生物。为完成这一工作,我们把幼虫的粪便直接接种在酸性培养基上,其中一些的pH值
From a number of literatures, the reports about the microbes in bee larvae are in conflict with each other. This may be due to research methods such as larval homogenates and larval gut homogenates; histological and microbiological techniques; selection of microbial culture media; and the use of diluents during larval homogenization, resulting in the isolation of small numbers of microorganisms in the gut of larvae The chance of reduction) sake. So the best way to isolate small quantities of microorganisms is again designed to study the intestinal microflora of larvae. To do this, we larvae faeces were directly inoculated on acidic medium, some of which pH