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采用 Tris-硼砂作为毛细管电泳分离测定甜菊苷的新体系 ,探讨了进样量、温度、电压、缓冲液浓度以及 p H值等条件对甜菊苷分离效果、灵敏度及分离时间的影响 .通过优化各种实验条件 ,建立一种毛细管区带电泳分离甜菊苷的新方法 .实验结果表明该方法具有如下优点 :1)快速 :甜菊中两种主要甜味成分在 3.5min内均达到完全分离 ;2 )灵敏度高 :最小检测浓度可达 6.2× 10 -5mol/ L;3)分离效率高 :理论塔板数高达 3.3× 10 4 / m;4 )稳定性好 :St出峰时间的 R.S.D为 0 .71% ,RA为0 .4 6% .Stevioside在 0 .5～ 7.0 mg/ m L的浓度范围内存在良好的线性关系 .实验实现了甜菊叶提取物中甜菊苷的分离测定 ,结果令人满意 .
Tris-borax as a capillary electrophoresis separation of stevioside new system, discuss the injection volume, temperature, voltage, buffer concentration and p H values and other conditions on the separation of stevioside effect, sensitivity and separation time by optimizing each The experimental results show that this method has the following advantages: 1) fast: the two main sweet components of Stevia are completely separated within 3.5 min; 2) High sensitivity: the minimum detection concentration of up to 6.2 × 10 -5mol / L; 3) high separation efficiency: the theoretical plate number up to 3.3 × 10 4 / m; 4) good stability: St peak time RSD was 0.71 %, And RA was 0.46% .Stevioside had a good linear relationship in the range of 0.5 ~ 7.0 mg / m L. The separation and determination of stevioside in stevia leaf extract were achieved experimentally and the results were satisfactory.