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小鼠血糖法测定胰岛素效价具有节省动物,设备简单,操作方便等优点,而且是量反应,反应的指标更接近临床,所以与小鼠惊厥法和兔血糖法比较有其优越性。现已被英国药典(1980版)所收载,我们也对此方法作了探讨,当时采用邻甲苯胺方法测定血糖值,但由于所用试剂有毒,而且用冰醋酸配制,对人和仪器都有损害,采血量也较多。据文献报导,葡萄糖氧化酶法测定血糖值,特异性较高,灵敏度高。前文曾报导用小鼠血糖法测
Mouse blood glucose determination of insulin titer with the advantages of saving animals, simple equipment, easy operation, but also the amount of reaction, the reaction is closer to clinical indicators, so with the mouse convulsive method and rabbit blood glucose method has its advantages. This method has been discussed in the United Kingdom Pharmacopoeia (1980 edition). At that time, the o-toluidine method was used to determine the blood glucose level. However, due to the toxicity of the reagents used and the preparation with glacial acetic acid, both human and apparatus Damage, blood volume is more. According to the literature, glucose oxidase method for the determination of blood glucose, high specificity and high sensitivity. The previous article reported the use of mouse blood glucose test