钌多吡啶配合物与DNA作用及抗肿瘤活性(英文)

• 作 者: 徐丽 陈禹 巫佳焕 闻伴康
• 机 构: 广东药学院医药化工学院,中山大学生命科学学院有害生物控制与资源利用国家重点实验室,
• 出 处: 无机化学学报
• 发表日期: 2004年期
• 关键词: DNA 细胞周期阻滞 钌配合物 抗肿瘤活性 插入方式 电子吸收光谱 细胞增殖 荧光光谱 断裂机理 粘度测定

应用电子吸收光谱、荧光光谱和粘度测定等方法研究钌多吡啶配合物[Ru(phen)2(Hecip)]2(phen=1,10-邻菲啰啉,Hecip=2(9-乙基-9H-咔唑-3-基)-1H-咪唑并[4,5-f][1,10]菲啰啉)与DNA相互作用。结果表明配合物与DNA键合计量比为2∶1,键合常数超过105mol-1.L,配合物以插入方式与DNA结合。运用琼脂糖凝胶电泳实验研究配合物诱导pBR322DNA断裂及断裂机理。体外抗肿瘤活性结果表明配合物能有效抑制肿瘤细胞增殖,进一步研究表明配合物可以将细胞周期阻滞在S期。

The electronic structure of Ru (phen) 2 (Hecip) 2 (phen = 1,10-phenanthroline, Hecip = 2) was studied by means of electron absorption spectroscopy, fluorescence spectroscopy and viscosity measurement. 9H-carbazol-3-yl) -1H-imidazo [4,5-f] [1,10] phenanthroline). The results showed that the ratio of complex to DNA bond was 2:1, and the binding constant was more than 105mol-1.L. The complex was inserted into DNA. The agarose gel electrophoresis was used to study the mechanism of pBR322 DNA cleavage and cleavage induced by the complex. In vitro antitumor activity results show that the complexes can effectively inhibit the proliferation of tumor cells, further studies show that the complex can block the cell cycle in the S phase.