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该研究采用ELISA法,对肝癌患者的血清检测HCV—Ab,用Nested-PCR法对患者的血清、肝癌组织和癌旁组织检测HCV-RNA和HCV-RNA负链,探讨肝细胞癌中是否存在HCV-RNA及其复制状态。对照组血清检测HCV-Ab、HCV-RNA1/17阳性。肝癌组16/31 HCV-RNA阳性;癌旁组织15例测到HCV-RNA负链,癌组织只有6例测到负链。结论:血清中抗HCV检测不能完全反映肝内是否曾有HCV感染情况。癌组织和癌旁组织均能检出HCV-RNA,说明肝癌组织中感染着丙型肝炎病毒。肝癌组织中确实存在HCV-RNA负链,说明HCV-RNA能在肝组织内不断地自我复制。
In this study, HCV-Ab was detected in sera of liver cancer patients by ELISA, and HCV-RNA and HCV-RNA negative strands were detected in serum, liver cancer tissues, and paracancerous tissues by Nested-PCR method to investigate the presence of hepatocellular carcinoma. HCV-RNA and its replication status. The serum of the control group was tested positive for HCV-Ab and HCV-RNA1/17. In the liver cancer group, 16/31 HCV-RNA was positive; in the paracancerous tissue, negative HCV-RNA was detected in 15 cases, and only 6 cases in the cancer tissue had negative chains. Conclusion: Anti-HCV testing in serum does not fully reflect whether there has been HCV infection in the liver. HCV-RNA can be detected in both cancer tissues and adjacent tissues, indicating that hepatitis C virus is infected in liver cancer tissues. The presence of HCV-RNA negative strands in HCC tissue suggests that HCV-RNA can continuously self-replicate in liver tissue.