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试验以经过高温筛选存活的海带配子体奔牛(Bn-23-b-2d,Bn-23-b-8d)和对照组配子体奔牛(Bn)为试验材料,在23℃分别处理4、8d和12d,进行了耐高温能力的验证。试验结果表明,3种材料的存活率随处理时间的延长均呈下降趋势,且3种材料的耐高温能力与之前的试验结果一致,依次为Bn-23-b-8d>Bn-23-b-2d>对照组Bn。同时,在高温处理的不同时间点(4、8、12、16d),对3种配子体的叶绿素a、脯氨酸、丙二醛及可溶性糖含量进行了检测,其中,对照组配子体叶绿素a含量随处理时间延长一直下降,而Bn-23-b-2d与Bn-23-b-8d有一定上升后下降;脯氨酸含量在高温胁迫下迅速积累,其中Bn-23-b-8d前期抵抗高温能力最强,抗高温时间最长;对照组Bn丙二醛和可溶性糖含量变化最明显,受到的高温胁迫强度最大。这4种生理指标的变化可以反映配子体的耐高温能力,与存活率验证得到的结果相符。该研究结果显示,高温筛选后其存活率和生理指标的变化能够反映其耐高温能力,为后期耐高温品种的选育奠定了基础。
In the present study, Bn-23-b-2d (Bn-23-b-8d) and gametophyte Benniu (Bn) 12d, to verify the ability of high temperature. The results showed that the survival rates of the three materials decreased with the increase of treatment time, and the high temperature resistance of the three materials was in good agreement with the previous ones, which were Bn-23-b-8d> Bn-23-b -2d> control group Bn. At the same time, the contents of chlorophyll a, proline, malondialdehyde and soluble sugar in the three gametophytes were detected at different time points (4, 8, 12 and 16 days). Among them, the content of chlorophyll a The proline content of Bn-23-b-2d and Bn-23-b-8d increased at a certain level and then decreased with the prolongation of treatment time. Proline content rapidly accumulated under high temperature stress, The strongest in high temperature and the longest in high temperature; the content of malondialdehyde and soluble sugar in Bn in control group was the most obvious, and the intensity of high temperature stress was the highest. The changes of these four physiological indexes can reflect the heat-tolerant capacity of gametophytes, which is consistent with the result of the survival rate verification. The results showed that the changes of survival rate and physiological index after high temperature screening can reflect the high temperature resistant ability, which lays the foundation for the breeding of late high temperature resistant varieties.