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以葡萄组培茎段为外植体,利用气升式生物反应器接触法培养,研究了不同蔗糖浓度对葡萄苗生长的影响,同时在栽培后调查成活率并观察气孔行为,以寻找一种简单、低成本及规模化培养优良葡萄苗的模式。结果表明:当蔗糖浓度为20g/L时苗的叶片颜色浓绿,节数多,苗和根的生长均良好。将反应器培养的葡萄苗分别移栽到蛭石和珍珠岩不同比率的移栽基质中,发现当蛭石量多时有利于葡萄苗的成活,单独使用蛭石的效果最佳,移栽30d后成活率达到100%;对反应器培养葡萄苗移栽过程中气孔进行观察,移栽后10d起部分气孔开始恢复关闭功能,14d后大部分(90%以上)气孔可以关闭,所以在对反应器葡萄苗进行移栽时,0~10d需进行保湿措施,14d以后可进行常规管理。
The stems of grape husks were used as explants and cultured with gas-lift bioreactor contact method. The effects of different sucrose concentrations on the growth of grape seedlings were studied. At the same time, the survival rate of the grape seedlings was investigated and the stomatal behaviors were observed to find a Simple, low cost and large-scale cultivation of good grape seedling mode. The results showed that when the sucrose concentration was 20g / L, the seedling leaves had dark green color, more nodes, and the growth of seedlings and roots were good. The grape seedlings cultured in reactor were transplanted into transplanting medium with different ratios of vermiculite and perlite respectively. It was found that when the amount of vermiculite was beneficial to the survival of grape seedlings, the effect of using vermiculite alone was the best. The rate reached 100%. The stomata during the transplanting of grape seedlings were observed. After 10 days from transplanting, the stomata started to recover and close, and most of stomata (90%) were closed after 10 days. Therefore, Seedlings for transplanting, 0 ~ 10d need to be moisturizing measures, after 14d can be routine management.