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目的:建立锝-99m(99mTc)标记寡核苷酸的方法,并用于家兔动脉粥样硬化斑块分子显像。方法:以高胆固醇饲料喂饲新西兰大耳白兔约2个月,复制成动脉粥样硬化家兔模型。寡核苷酸进行99mTc标记后注入家兔,进行生物学分布以及体内、体外显像研究。结果:寡核苷酸99mTc标记率为(77.8±6.8)%(n=7)。生物学分布实验表明,与SON相比,c-mycASON在粥样斑块中的浓聚程度明显要高。c-mycASON体内显像可见腹主动脉部位散在性显像剂浓聚,体外主动脉显像提示放射性浓聚灶与斑块所在部位吻合。未偶联组未见阳性显像结果。结论:99mTc标记反义寡核苷酸有望成为一种新的显像剂,在分子水平上用于动脉粥样硬化的早期、特异和无创性的诊断。
Objective: To establish a technetium-99m (99mTc) labeled oligonucleotide and to use in the molecular imaging of atherosclerotic plaque in rabbits. Methods: New Zealand white rabbits were fed with high cholesterol diet for about 2 months and then made into atherosclerotic rabbit model. Oligonucleotides were labeled with 99mTc and then injected into rabbits for biological distribution as well as in vivo and in vitro imaging studies. Results: The 99mTc labeling rate of oligonucleotide was (77.8 ± 6.8)% (n = 7). Biodistribution experiments showed that, compared with SON, c-mycASON in atherosclerotic plaque was significantly higher concentration. In vivo imaging of c-mycASON visible scattered abdominal aortic imaging agent concentration, in vitro aortic imaging prompted radioactive focal lesions and plaque site consistent. Unconjugated group no positive imaging results. CONCLUSIONS: The 99mTc labeled antisense oligonucleotide is expected to be a new imaging agent for the early, specific and noninvasive diagnosis of atherosclerosis at the molecular level.