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目的 探讨因吡喹酮抗性产生而导致曼氏血吸虫抗性虫株繁殖力发生改变的可能性。方法 以抗性株与敏感株毛蚴和尾蚴定量感染光滑双脐螺和CD1小鼠,宿主体内观察比较曼氏血吸虫吡喹酮抗性株和敏感株生物学特性:对螺感染率、感染性螺存活期、在螺体内产尾蚴量、尾蚴和虫卵开放前期及虫卵孵化率。结果 单只螺暴露于单个毛蚴2h,抗性株与敏感株对螺感染率为19.8％和8.9％,两者间差异有非常显著性(P=0.006);逸蚴期间,塞内加尔抗性株感染螺存活率高于敏感株;用塞内加尔抗性株和敏感株毛蚴感染所获阳性螺产尾蚴量(尾蚴/螺)为6875条和14611条;塞内加尔抗性株和敏感株尾蚴开放前期为32.2d和27.7d。抗性株与敏感株鼠体内虫卵开放前期为42.8d和45.5d;虫卵孵化率为80.9％和78.O％。结论 曼氏血吸虫吡喹酮抗性株对实验室传代的光滑双脐螺具有高感染性,此生物学改变似与吡喹酮抗性产生相关;塞内加尔抗性株与敏感株间存在其他变化则被认为是曼氏血吸虫不同地理株间的差异。
Objective To explore the possibility of the change of fecundity of Schistosoma mansoni strains caused by praziquantel resistance. Methods The biological characteristics of praziquantel-resistant and sensitive strains of Schistosoma mansoni were observed and compared in vivo by quantitative detection of miracidia and cercariae. Survival, the amount of cercariae produced in the spirochete, cercariae and egg pre-opening and hatching rate of eggs. Results The results showed that the infection rates of single strain of spirochete larvae were only 2h, the rate of infection was 19.8% and 8.9% respectively between the resistant and susceptible strains, the difference was significant (P = 0.006); Senegal resistant strain was infected Survival rate of spirochaeta was higher than that of the susceptible strain. The numbers of positive cercariae (cercariae / cercariae) were 6875 and 14611, respectively. Senegal-resistant strains and sensitive cercariae were 32.2 days And 27.7d. The resistant strains and sensitive strains of mice preeclampsia eggs 42.8d and 45.5d; hatching rate of eggs 80.9% and 78. O%. Conclusion The praziquantel-resistant strains of Schistosoma mansoni were highly infective to the smooth-bodied smooth-handed Mirabilis isolates from the laboratory, and the biological changes seemed to be related to the development of praziquantel resistance. There were other changes in Senegal-resistant and susceptible strains It is considered as a difference between different geographic strains of Schistosoma mansoni.