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为了研究华山新麦草染色体在小麦-华山新麦草各衍生世代中的遗传规律并建立一套小麦-华山新麦草异源附加系,对小麦-华山新麦草BC1F2到BC1F5共315个植株进行细胞学检测和GISH分析,结果表明,染色体数目分布范围为40~54,2n≥43的植株有223株,占总观察株数的70.79%;早代植株携带华山新麦草染色体的概率较高,植株染色体数目多大于44,随着自交代数的增加,外源染色体丢失的概率也在增大。因而在选育小麦-华山新麦草异附加系时,在BC1F4和BC1F5世代选择效果较好;通过对染色体数目2n=44的42个植株进行GISH分析,筛选出39个二体附加植株。
In order to study the genetic rules of P. huashanica chromosome in wheat-huashanqing wheat and to establish a wheat-huashanica heterologous addition line, 315 plants from wheat BC1F2 to BC1F5 And GISH. The results showed that there were 223 plants with the number distribution of 40 ~ 54 and 2n≥43, accounting for 70.79% of the total observed. The early plants had a higher probability of carrying chromosomes of P. huashanica and more chromosomes Above 44, the probability of exogenous chromosome loss is also increasing as the number of selfing crosses increases. Therefore, selection of BC-F4 and BC1F5 generations was more effective when wheat-Huashanica alien addition lines were selected. By means of GISH analysis of 42 plants with chromosome number 2n = 44, 39 plants were selected.