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目的了解2005-2007年吉林省乙型流行性感冒(流感)病毒HA1基因的演变特征。方法采用MDCK分离培养流感病毒,提取病毒RNA,经反转录和聚合酶链式反应扩增后测序。结果2005-2007年吉林省同时流行着乙型Victoria系和Yamagata系流感毒株。与B/Shanghai/361/02相比,2005年流行的乙型Yamagata系病毒有8个氨基酸位点发生改变;2007年流行的乙型Yamagata系病毒有10个氨基酸变化位点;2007年流行的乙型Victoria系毒株与B/Malaysia/2506/04相比,只有3个氨基酸位点发生替换。结论2005-2007年吉林省流行的乙型Yamagata系病毒与B/Shanghai/361/02相比已经发生变化;吉林省2007年Victoria系毒株与疫苗代表株B/Malaysia/2506/04比较接近。
Objective To understand the evolution of HA1 gene of influenza B (influenza) virus in Jilin province from 2005 to 2007. Methods The influenza virus was isolated and cultured by MDCK. The viral RNA was extracted and amplified by reverse transcription and polymerase chain reaction (PCR). Results From 2005 to 2007, the strains of influenza B of Victoria Victoria and Yamagata were prevailed in Jilin Province at the same time. Compared with B / Shanghai / 361/02, there were 8 amino acid changes in the Yamagata B virus in 2005. There were 10 amino acid changes in the Yamagata B virus in 2007, Compared with B / Malaysia / 2506/04, only the three amino acid substitutions were found in type B Victoria strain. Conclusions The prevalence of type B Yamagata virus in Jilin Province from 2005 to 2007 has been changed compared with that of B / Shanghai / 361/02. In 2007, Victoria strain and vaccine strain B / Malaysia / 2506/04 in Jilin Province were relatively close.