Immobilization of Aminoacylase From Aspergillus oryzae on Functionalized Acrylamide-Methylacrylate C

• 作 者: 王道宾 周菊岩 李民勤 何炳林
• 机 构: Institute of Polymer Chemistry, Nankai University, Tlanjin 300071, PRC,Institute of Polymer Chemistr
• 出 处: Progress in Natural Science
• 发表日期: 1994年02期
• 关键词: amination polyethylene polymeric methionine operational beads suspensions immobi

Poly (acryiamide-co-toethyi acryiate) beads have been synthesized by oil phase suspensions. These polymers were functionalized by amination with diamines or polyethylene polyamines in order to be applied to the immobilization of aminoacylase from Aspergillus oryzae. The influence of polymeric structural property on the activity of immobilized enzyme was investigated. A good carrier (IAR-2) was elected for the preparation of immobilized enzyme and many factors which affected the activity of immobilized enzyme were described. The operational stability for continuous resolution of DL-methionine on the immobilized enzyme column was also studied.

Poly (acryiamide-co-toethyi acryiate) beads have been synthesized by oil phase suspensions. These polymers were functionalized by amination with diamines or polyethylene polyamines in order to be applied to the immobilization of aminoacylase from Aspergillus oryzae. The influence of polymeric structural properties on the activity of immobilized enzyme was investigated. A good carrier (IAR-2) was elected for the preparation of immobilized enzyme and many factors which affected the activity of immobilized enzyme were described. The operational stability for continuous resolution of DL-methionine on the immobilized enzyme column was also studied.