【摘 要】
：
The 20th anniversary of the completion of the Human Genome Project offers an opportunity to reflect on early efforts to make biological sense out of genomic data.My interest in genomic analysis began at Washington University in 1988 while I was a postdoc
【机 构】
：
The Institute for Genome Sciences and Department of Biochemistry & Molecular Biology,University of M
论文部分内容阅读
The 20th anniversary of the completion of the Human Genome Project offers an opportunity to reflect on early efforts to make biological sense out of genomic data.My interest in genomic analysis began at Washington University in 1988 while I was a postdoc in Doug Berg\'s laboratory,which was in the same building as the laboratory of Maynard Olson,one of the founders of the Human Genome Project.At the time,my focus was on the molecular biology of E.coli-an area of research that had appealed to me due to its being information rich.However,other,emerging areas of research began to offer great opportunities going forward.At an inhouse seminar,Maynard Olson made a visionary statement foreseeing the day when each patient\'s genome would be sequenced as part of a routine medical exam.After having spent years as a graduate student sequencing a mere 5,000 base pairs using the Maxam and Gilbert method,Maynard\'s statement initially struck me as incredible,but later as eye opening.The whole genome analysis initiative also raised the question of how to best obtain biological information from the vast amount of sequence data becoming available.This directed me toward computer science and statistcs,which were soon to become very critical components of biomedical research.
其他文献
Rett syndrome (RTT) is a progressive neurodevelop-mental disorder,mainly caused by mutations in MeCP2 and currently with no cure.We report here that neurons from R106W MeCP2 RTT human iPSCs as well as human embryonic stem cells after MeCP2 knockdown exhib
Chaperone-mediated autophagy (CMA) is a lysosome-dependent selective degradation pathway implicated in the pathogenesis of cancer and neurodegenerative dis-eases.However,the mechanisms that regulate CMA are not fully understood.Here,using unbiased drug sc
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is the fourth-leading cause of cancer-related deaths worldwide.HCC is refractory to many standard cancer treatments and the prognosis is often poor,highlighting a pressing need
Oxygen,iron,and polyunsaturated fatty acids (PUFAs;fatty acids containing more than one double bond) are all bene-ficial to our cellular lives.Incorporation of these components into cellular processes,however,comes at a cost:the bis-allylic structure of P
Background:Commitment to a new cell cycle is controlled by a number of cellular signals.Mitogen-activated protein kinase (MAPK) pathways,which transduce multiple extracellular cues,have been shown to be interconnected with the cell cycle and can modulate
The evaluation of simulated disasters (for example,exercises) and real responses are important activities.However,little attention has been paid to how reports documenting such events should be written.A key issue is how to make them as useful as possible
Background:The core concept of cell engineering is the use of synthetic biological methods to engineer and design genetically modified cells with specific functions,which has revolutionized the biotechnology industry and cell therapy.Implanted cells play
Quick response research conducted by social scientists in the aftermath of a disaster can reveal important findings about hazards and their impacts on communities.Research to collect perishable data,or data that will change or be lost over time,immediatel
Floods are among the most frequent and devas-tating natural hazards and disasters in many southern states in the United States.This study examined the relationship and reciprocal predictability between two theoretical con-structs—risk perception attitude
The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),which is spread primary via respiratory droplets and infects the lungs.Currently widely used cell lines and anima