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目的分析RCAS1在再生障碍性贫血患者骨髓上清及骨髓基质细胞培养上清中的表达。方法酶联免疫吸附试验(ELISA)方法测定17例再生障碍性贫血患者及18例正常对照骨髓上清及骨髓基质细胞培养上清中sRCAS1水平。结果①再生障碍性贫血患者骨髓上清中sRCAS1水平为(0.37±0.14)μg/L,正常对照为(0.09±0.11)μg/L(P<0.05)。②再生障碍性贫血患者基质细胞培养上清中sRCAS1水平为(1.00±0.08)μg/L,正常对照组为(0.81±0.05)μg/L(P<0.05)。结论再生障碍性贫血患者骨髓上清及骨髓基质细胞培养上清中sRCAS1水平明显高于正常对照组。
Objective To analyze the expression of RCAS1 in the supernatant of bone marrow and bone marrow stromal cells of patients with aplastic anemia. Methods The levels of sRCAS1 in 17 cases of aplastic anemia and 18 cases of normal control bone marrow stromal cells and supernatant of bone marrow stromal cells were measured by enzyme linked immunosorbent assay (ELISA). Results ① The levels of sRCAS1 in the bone marrow of patients with aplastic anemia were (0.37 ± 0.14) μg / L and (0.09 ± 0.11) μg / L, respectively (P <0.05). ② The level of sRCAS1 in the culture supernatant of aplastic anemia was (1.00 ± 0.08) μg / L and that of the normal control group was (0.81 ± 0.05) μg / L (P <0.05). Conclusion The level of sRCAS1 in bone marrow supernatant and bone marrow stromal cell culture supernatant of patients with aplastic anemia was significantly higher than that of the normal control group.