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收集１０例正常胃粘膜，１８例慢性萎缩性胃炎Ａ型（ＣＡＧ－Ａ），２４例慢性萎缩性胃炎Ｂ型（ＣＡＧ－Ｂ）活检标本。应用免疫组织化学方法及图像分析定量技术对胃粘膜神经内分泌（ＮＥ）细胞进行定量研究。第一抗体包括抗－嗜铬粒蛋白Ａ、五羟色胺、组织胺、胃泌素、生长抑素、胰多肽、胰高血糖素、绒毛膜促性腺激素、促肾上腺激素共９种。结果显示两型萎缩性胃炎的ＮＥ细胞数量及种类的变化均有不同。正常胃窦ＮＥ细胞计数为（１４５±９）／ｍｍ２，胃体为（１３８±１２）／ｍｍ２。在ＣＡＧ－Ａ中，胃窦及胃体ＮＥ细胞明显增生，分别为（２５６±２１）／ｍｍ２，（２７８±１３）／ｍｍ２（Ｐ＜０．０１）。ＣＡＧ－Ｂ中胃窦与胃体ＮＥ细胞明显减少，分别为（６９±７）／ｍｍ２（Ｐ＜０．０１）及（１０９±８）／ｍｍ２（Ｐ＜０．０５），并出现在正常胃粘膜没有的胰高血糖素阳性细胞。提示两型萎缩性胃炎ＮＥ细胞变化在其发病中可能有着重要作用。
Ten normal gastric mucosa, 18 chronic atrophic gastritis type A (CAG-A) and 24 chronic atrophic gastritis type B (CAG-B) biopsy specimens were collected. Gastric mucosal neuroendocrine (NE) cells were quantitatively studied by immunohistochemistry and image analysis. The first antibody includes anti-chromogranin A, serotonin, histamine, gastrin, somatostatin, pancreatic polypeptide, glucagon, chorionic gonadotropin, adrenocorticotropic hormone were a total of 9 species. The results showed that two types of atrophic gastritis NE cell number and type of changes are different. Normal gastric antral NE cell count was (145 ± 9) / mm2, gastric body (138 ± 12) / mm2. In CAG-A, gastric neoplasms and gastric neoplasms were significantly hyperplastic (256 ± 21) / mm2 and (278 ± 13) / mm2, respectively (P <0.01). The number of NE cells in gastric antrum and gastric body in CAG-B decreased significantly (69 ± 7) / mm2 (P <0.01) and (109 ± 8) / mm2 respectively (P <0.05) Glucagon-positive cells not present in the gastric mucosa. Tip two types of atrophic gastritis NE cell changes in the pathogenesis may play an important role.