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目的:优选长叶胡颓子总三萜酸的大孔吸附树脂纯化工艺。方法:采用UV测定总三萜酸含量。通过动态吸附-洗脱试验筛选大孔树脂型号;以总三萜酸含量为指标,通过单因素试验筛选纯化工艺的影响因素,Plackett-Burman试验筛选主要影响因素。以总三萜酸含量和浸膏得率的综合评分为指标,选取乙醇体积分数、洗脱剂用量、醇碱比为考察因素,通过正交试验优选长叶胡颓子总三萜酸的富集工艺。结果:长叶胡颓子总三萜酸的最佳纯化工艺为洗脱剂用量4 BV,醇碱比1∶5,乙醇体积分数80%,洗脱流速3 mL·min-1;总三萜酸质量分数达75%,浸膏收率达22%。结论:利用Plackett-Burman试验设计联用正交试验优选的纯化工艺稳定、简便易行,为长叶胡颓子三萜酸的开发利用提供实验依据。
Objective: To optimize the purification process of macroporous adsorption resin of total triterpenes from Elaeagnus. Methods: Total triterpene acid content was determined by UV. The macroporous resin model was screened by dynamic adsorption-elution test. The influencing factors of purification process were screened by single factor test with total triterpene acid content as the index. The main influencing factors were screened by Plackett-Burman test. Taking the total score of total triterpene acid content and extract yield as indexes, the ethanol volume fraction, the amount of eluent and the ratio of alkalis to alkalis were selected as the investigation factors. Orthogonal test was conducted to optimize the total triterpene acid content Set technology. Results: The optimal purification technology of total triterpene acid was 4 BV, alcohol-base ratio 1: 5, ethanol volume fraction 80% and elution flow rate 3 mL · min-1. Total triterpene Acid mass fraction of 75%, extract yield of 22%. Conclusion: The optimized purification process by Plackett-Burman design combined with orthogonal experiment is simple, easy and convenient, and provides an experimental basis for the exploitation and utilization of Elaeagnus bracteatum.